Page 186 - Applied Pharmacognosy
P. 186

Detectors in HPLC

                     UV Derivatization Reagents

❑ If sample is non-UV absorbing, sample derivatization is needed.
❑ It can be either pre-column or post-column derivatization.

                      Reaction  185
                      chamber

          Pump
Reaction Post-Column
solution Derivatization
   181   182   183   184   185   186   187   188   189   190   191