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Detectors in HPLC

                     UV Derivatization Reagents

❑ UV-absorbing groups can be covalently attached to the analyte.

          DNBC Reagent (Reacts with Amines & alcohols)

3,5-dinitrobenzoyl chloride
              DNBC

PNBA Reagent (Reacts with Aldehydes & ketones)

p-Nitrobenzyloxyamine hydrochloride  186
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