Page 189 - Applied Pharmacognosy
P. 189

Detectors in HPLC

                    Fluorescence Detector

❑ Its use is restricted to compounds that fluoresce naturally

such as polyenes and aromatics (have fluorophore group).

Xenon lamp

                    Fluorescence
                        grating

                                                Photomultiplier tube

                                  Fluorescence

Excitation grating  Excitation Sample cell
                        light

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