Page 191 - Applied Pharmacognosy
P. 191
Detectors in HPLC
Evaporative Light Scattering Detector (ELSD)
❑ ELSD responds to any analyte that is significantly less volatile
than the mobile phase (it allows gradient elution).
❑ Used for analytes that have poor to no UV/Vis response.
Evaporation Light-receiving unit or Photodetector
Nebulization Heated drift tube
Nebulizer Detection
Column eluate
Nebulizer gas Assist gas
Drain
Laser Light source
The column eluate is evaporated and the light scattered by the
particles of nonvolatile substances is detected. 190
