Page 190 - Applied Pharmacognosy
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Detectors in HPLC
Fluorescence Derivatization Reagents
❑ If sample is non-fluorescent, sample derivatization is needed.
❑ Fluorescent groups can be covalently attached to the analyte.
OPA Reagent (Reacts with Primary Amines)
CHO S-R’
+ R-NH2 R’-SH N-R
CHO
o-phthalaldehyde (OPA)
ADAM Reagent (Reacts with Fatty Acids)
+ R-COOH CH2OCOR189
CHN2
9-anthryldiazomethane (ADAM)
