Page 21 - D:\Video IPSyoFS22\

Evaluation of antioxidant properties of leaves and barks extracts of Neolamarckia cadamba

1 2 3 1,4 *
Nur Arina Nuha Abdul Nasir, Yosie Andriani, Maulidiani M, Aidilla Mubarak

1 Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu,
21030 Kuala Nerus, Terengganu, Malaysia.
2 Institute of Marine Biotechnology (IMB), Universiti Malaysia Terengganu,
21030 Kuala Nerus, Terengganu, Malaysia.
3 Faculty of Science and Marine Environment, Universiti Malaysia Terengganu,
21030 Kuala Nerus, Terengganu, Malaysia.
4 Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu,
21030 Kuala Nerus, Terengganu, Malaysia.

* Corresponding author: aidilla@umt.edu.my

Abstract:
The medicinal plant has been an evident natural source of phytochemicals such as polyphenols that
provides many therapeutic values. The remedial effects of the plants have been largely attributed to the
antioxidant values. Some plants discovered in Malaysia are still considered to be underexplored, which
include Kadamba or Kelampayan (Neolamarckia cadamba). Multiple parts of its plants like barks,
leaves, fruits and flowers have been used as a source of ethnomedicine to treat various illnesses.
However, the information on the phenolics and antioxidant values of these plants is limited. Leaves and
barks of Neolamarckia cadamba obtained from Mentakab, Pahang were extracted using an ultrasonic-
assisted methanolic extraction method, and the total phenolic content (TPC) was determined by the
Folin-Ciocalteu method. The antioxidant capacity of the plant extracts was measured by 2,2-diphenyl-
1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP).
Extract of N. cadamba bark showed a higher value of TPC (58.00 ± 0.31 mg GAE/g dry extract weight
than the leaves extract (41.00 ± 0.36 mg GAE/g dry extract weight). The inhibitory concentration (IC50)
values of N. cadamba leaf and bark extracts were measured and compared with the synthetic
antioxidants, BHT and Trolox. The order of antioxidant DPPH free radical scavenging activity based
on their respective IC50 value obtained was Trolox>Barks>Leaves>BHT. The FRAP values for leaves
and barks extracts are in the range of 24 ± 0.936 μmol FeSO4/g DW to 72 ± 0.422 μmol FeSO4/g DW
where barks extract recorded a higher value than leaves extract. Barks extract showed higher antioxidant
activity than leaves extract which promises potential in the nutraceutical industry.

Keywords: Medicinal plants, Ethnomedicine, Neolamarckia cadamba, Polyphenols, Antioxidant