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11. Expel any air from the syringe and then cap the needle. Caution. Leave the cap on the
table and thread the needle into it to avoid accidental needle puncture
12. Gently roll the syringe between the palms of your hands to mix the heparin with the
blood.
13. Place the syringe in ice and send the specimen immediately to the lab for analysis.
14. Either you or the patient should keep applying pressure to the vessel for a few minutes.
Then apply a Band-Aid and the procedure is complete

13.3 Taking sputum specimen
Objective: At the end of this lesson, the learner will able to:
• Assemble necessary equipment
• Demonstrate the proper technique of taking sputum specimen
• Interpret the result after taking the sputum specimen
• Demonstrate proper handling of the sputum sample
Definition: is a method of collection of coughed sputum for the diagnostic purpose (to
rule out respiratory pathology).
Purpose: Examination of sputum may aid in the diagnosis and treatment of several
conditions ranging from simple bronchitis to lung cancer.
Three major types of sputum specimens:
Cytology- cytology or cellular examination of sputum may identify aberrant cells or
cancer.
Culture and sensitivity – used to identify specific microorganisms and to determine
antibiotics to which they are most sensitive.
Acid-fast bacilli (AFB)-used to support the diagnosis of tuberculosis (TB)
Suctioning may be indicated to collect sputum from the client who is unable to
spontaneously produce a sample for laboratory analysis.
Suctioning may provoke violent coughing, which can induce constriction of pharyngeal,
laryngeal, and bronchial muscles.

Equipment
1. Sterile specimen container with a cover
2. Clean disposable gloves
3. Facial tissue
4. Emesis basin (optional)
5. Toothbrush (optional)
6. Completed identification label
7. Completed laboratory request

Procedure
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