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human literature. Once this bullseye effect is appre-  The abdominal fluid score (AFS) has been devel-
             ciated, the operator may cannulate the vessel or   oped to predict the severity of anemia in the trau-
  VetBooks.ir  collect the blood sample.                 matic hemoabdomen by differentiating between a
               For  longitudinal orientation venipuncture, the
                                                         small and large bleeding event. The same AFS sys-
             operator should start by identifying and focusing
                                                         rhage serial monitoring.  The  AFS allows for
             on the image of the vessel in transverse orientation.   tem has also been applied in postoperative hemor-
             Then, the US probe is rotated 90° so that the vessel   semi-quantification of the degree of hemorrhage for
             appears as an anechoic to hypoechoic tract across   comparison at different time points and may allow
             the screen with the probe indicator/notch facing   veterinarians to predict the future need for blood
             rostrally. The needle or introducer catheter should   products. This scoring system has been validated in
             be held within the dominant hand and placed   cases of trauma in dogs but is not predictive for
             through the skin 1–2 cm rostral to the US probe   anemia and transfusion requirements in cats.
             and perpendicular to the indicator/notice on the   An AFS of 1–2 is associated with small amounts
             transducer. The angle that the needle or introducer   of bleeding, and an AFS of 3–4 is associated with
             catheter should be inserted into the skin is depend-  larger bleeding events. In dogs, higher AFS scores in
             ent  upon  the  depth  of  the  vessel  and  distance  of   trauma cases are associated with development of
             insertion rostral to the US probe.          anemia, a higher need for blood transfusions, and,
               Once the needle or introducer catheter is inserted   rarely, a requirement for surgical intervention. In
             into the skin, the US probe is moved slightly ros-  trauma patients, the  AFS can also help a practi-
             trally to visualize the hyperechoic needle as it   tioner to determine sources of blood loss. For
             inserts into the vessel through the dorsal vessel wall   example, if a patient without a pre-existing anemia
             (Fig. 7.9B). The vessel wall may bow inward or roll   that has a static traumatic AFS of only 1–2 devel-
             away from the US view. If this occurs, the operator   ops anemia in the hospital, a clinician should look
             should slightly withdraw the needle or introducer   for other sources of ongoing hemorrhage including,
             catheter and optimize the image of the vessel before   but not limited to, the pericardial space, pleural
             trying again. When the vessel has been successfully   space, pulmonary parenchyma, and fracture sites.
             penetrated, the bowing of the vessel should resolve   Clinically, the AFS has been also utilized for scor-
             and a flash of blood into the catheter or needle   ing the accumulation of other non-traumatic abdom-
             should be seen.                             inal effusions. If the effusion amount is increasing, it
               5.  If the operator is using the UST for vascular   indicates a need for further diagnostic or therapeutic
             access to achieve a central venous catheter, once vas-  interventions. These effusions include non-traumatic
             cular penetration has been confirmed, the modified   bleeding (ruptured masses, coagulopathies, and post
             Seldinger technique (guidewire-based technique) can   interventional hemorrhage), urine, septic effusion,
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             be performed for either approach. The US can be used   and  non-septic  effusion. The AFAST  can be more
             to identify if a guidewire is feeding into the vessel   sensitive in detecting hemorrhage than serial packed
             appropriately during that step of the Seldinger tech-  cell volume/total solids (PCV/TS), physical examina-
             nique. See Further Reading section for a more detailed   tion findings, and radiographs since there is a great
             description of the modified Seldinger technique.  ability to compensate for blood loss. Humans with
                                                         ongoing blood loss have been noted to compensate
                                                         with normal vital parameters until nearly 30% total
             7.3  Indications for Ultrasound             blood loss has occurred.
             Evaluation
             AFAST /Abdominal Fluid Score
                   3
                                                               3
                                                         TFAST /VetBLUE
             Since its first description in veterinary medicine in
             2004 and validation in 2009, AFAST  has multiple   Use  of  TFAST   in  veterinary medicine  was  first
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             applications (Table 7.3). It has been shown to be   documented in 2008 for detection of pneumotho-
             superior to physical examination alone and more   rax (Lisciandro, 2008) in trauma patients.  This
             sensitive than abdominal radiographs for small   study found that US by an experienced operator
             amounts of effusion (up to 24% of patients with   had a sensitivity of 95% for detecting pneumotho-
             normal abdominal radiographs have been shown   rax. Since then, the  TFAST  has been utilized to
                                                                               3
                                      3
             to have fluid present on AFAST  examination).  evaluate the intrathoracic organs and thoracic
             Applications of Serial Focal Ultrasound Techniques in the Hospitalized Small Animal Patient   145
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