Page 23 - Live-cellanalysis handbook

Kinetic Proliferation Assays

Figure 5. 384-well microplate view of HT-1080 NucLight Green cell proliferation with 16 different compounds, 11-point concentration-response curves in
duplicate (different colors, high to low concentrations left to right). Columns 15 and 16 are vehicle (0.5% DMSO) and CHX (3 μM) controls, respectively.
Note the potent concentration-dependent inhibition of cell proliferation for certain compounds (e.g. row J, row M, row O), and weaker effects/inactivity
of others (e.g. row A, row P). Abscissa: time (0-72h), ordinate: fluorescent object count per well (0-3800).
Maximum object count (1/mm ) 2
TAME PD98059 Compound 401 10-DEBC FAK Inhibitor 14 FPA124
4000
3000
2000
1000

0
-8 -6 -4 -8 -6 -4 -8 -6 -4 -8 -6 -4 -8 -6 -4 -9 -7 -5
KU0063794 Cycloheximide Chrysin Mitomysin C Staurosporin RITA
4000
3000
2000
1000

0
-10 -8 -6 -10 -8 -6 -7 -5 -3 -8 -6 -4 -10 -8 -8 -8 -6 -4v
Doxorubicin Cisplatin Camptothecin PAC1
4000
Figure 6. Concentration-response curves for a
3000 selected set of literature standard inhibitors of
cell proliferation in HT-1080 NucLight Green
2000 cells. Abscissa: -log M [compound], ordinate:
maximum nuclear count (per mm ). Each point
2
1000 represents the average data from two wells from
a single 384-well compound plate. The lines of
0 best fit are a 4-parameter logistic equation and
-8 -6 -4 -8 -6 -4 -8 -6 -4 -11 -9 -7 can be calculated using IncuCyte software.
Log[compound] (M)

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